CJC-1295 with DAC
Tetrasubstituted GHRH Agonist & Drug Affinity Complex – HPLC Verified
Comprehensive Product Description
CJC-1295 with DAC is a synthetic, tetrasubstituted analogue of Growth Hormone-Releasing Hormone (GHRH) modified with a Drug Affinity Complex (DAC). mindpeptides synthesizes this advanced peptide to address the rapid degradation limitations of native GHRH (1-29). By incorporating four specific amino acid substitutions (D-Ala, Gln, Ala, Leu) and a maleimidopropionic acid linker (DAC), this research compound demonstrates exceptional resistance to enzymatic cleavage by DPP-IV.
The defining feature of this product is the DAC moiety, which facilitates a bioconjugation reaction with serum albumin in vivo. This binding event significantly expands the hydrodynamic volume of the peptide, preventing renal clearance and extending the plasma half-life from minutes to several days. CJC-1295 DAC is established as a premier tool for researchers investigating sustained growth hormone (GH) pulsatility and downstream Insulin-like Growth Factor 1 (IGF-1) elevation without the need for frequent administration associated with unmodified secretagogues.
Mechanism of Action & Signaling Pathways
Albumin Bioconjugation & Somatotroph Activation
Upon introduction to the research substrate, the maleimide group of the DAC moiety forms a stable covalent thioether bond with the free thiol group of Cysteine-34 on serum albumin. This complex acts as a circulating reservoir, protecting the GHRH analogue from proteolysis while allowing it to bind to GHRH receptors on anterior pituitary somatotrophs.
Once bound to the receptor, the peptide activates the Gs-protein coupled pathway, stimulating adenylate cyclase and increasing intracellular cAMP. This signaling cascade leads to the phosphorylation of CREB and subsequent transcription of GH genes. The result is a sustained, physiological-like amplification of basal and pulsatile GH secretion, which subsequently drives hepatic synthesis of IGF-1, a critical mediator of cellular growth and metabolic regulation.
Expanded Research Applications
- Extended Pharmacokinetics: Utilized in studies comparing short-acting vectors (like Mod GRF 1-29) against long-acting albumin-bound complexes to understand half-life dynamics and receptor desensitization thresholds.
- IGF-1 Axis & Metabolism: A vital tool for observing the effects of chronically elevated IGF-1 levels on muscle protein synthesis, lipolysis, and glucose metabolism in animal models.
- Pituitary Function Modeling: Used to assess the secretory capacity of the pituitary gland over extended durations, providing data on pulse amplitude versus frequency in controlled laboratory environments.
Laboratory Reconstitution Guidelines
mindpeptides supplies CJC-1295 DAC as a sterile lyophilized powder requiring careful reconstitution for stability.
- Solvent: Bacteriostatic water is required. The lyophilization process ensures stability at room temperature prior to mixing.
- Procedure: Inject solvent gently down the vial wall. Allow the “puffy white powder” (lyophilized cake) to dissolve without vigorous shaking to preserve the peptide structure.
- Handling: Once reconstituted, the solution is stable for up to 30 days if kept refrigerated (4°C). For long-term storage of dry powder, -80°C is optimal.
Reviews
There are no reviews yet.